Summary
A fully representative and viable platelet population was isolated from the blood
of 15 baboons by a multiwash procedure, and labelled with In-lll-oxine. The recovery
of the total platelet population in the circulation was 85% ± 9. Mean platelet life
span was 146 hr ± 13. Correcting for plasma radioactivity (always less than 3.5%)
did not significantly affect the estimate of platelet life span (145 hr ± 16) or recovery
(85% ± 12). Platelet survival estimates, repeated at different times, were reproducible.
In 5 baboons, platelets were also harvested by a single step differential centrifugation.
The mean life span of a representative platelet population was significantly longer
than that of platelets harvested by a single step. Recovery values of the representative
and non-representative population were similar. We conclude that it may be important
to harvest and label a fully representative platelet population for kinetic studies.
The proposed method is simple and reproducible, and may be applied in studies in humans.
Keywords
Indium-111 - Primates - In-lll-labelled platelets - Platelet labelling technique -
Blood platelets
